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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas Multiplexed Biosensing: Advances, Challenges, and Perspectives.

Luyu Wei1, Zhilong Wang2, Yuanbin She3

  • 1The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China.

Analytical Chemistry
|May 27, 2025
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This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems offer sensitive disease diagnostics. Recent advances in CRISPR/Cas multiplexed biosensing overcome signal interference, enabling reliable detection of multiple targets.

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Genomics

Background:

  • CRISPR/Cas systems are highly sensitive and specific tools for molecular diagnostics.
  • Multiplexed detection of multiple targets is crucial for accurate disease diagnosis.
  • Nonspecific trans-cleavage has historically limited multiplexed CRISPR/Cas applications.

Purpose of the Study:

  • To systematically review recent advancements in CRISPR/Cas-powered multiplexed biosensing strategies.
  • To analyze the advantages, limitations, and sensing mechanisms of various CRISPR/Cas multiplexed detection methods.
  • To discuss the current status, challenges, and future outlook of CRISPR/Cas multiplexed biosensing.

Main Methods:

  • Review of recent scientific literature on CRISPR/Cas multiplexed detection systems.
  • Focus on Cas9, Cas12, and Cas13 systems.
  • Analysis of validated examples, sensing mechanisms, and detection performance.

Main Results:

  • Significant progress in CRISPR/Cas multiplexed biosensing strategies over the past five years.
  • Development of methods to overcome signal interference in multiplexed detection.
  • Identification of key challenges and opportunities for practical application.

Conclusions:

  • CRISPR/Cas multiplexed biosensing has rapidly advanced, addressing previous limitations.
  • Further research is needed to optimize strategies for widespread clinical application.
  • The field holds significant potential for next-generation disease diagnostics.