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Updated: Sep 20, 2025
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Enhanced abscisic acid detection via SERS-active single crystal MAPbCl3 nanofiber-based Self-CoAptaNano (SCAN)
Muhammad Awais1, Syed Muhammad Zaigham Abbas Naqvi1, Yanyan Zhang1
1College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China; Henan International Joint Laboratory of Laser Technology in Agriculture Sciences, Zhengzhou, 450002, China; State Key Laboratory of Wheat and Maize Crap Science, Zhengzhou, 450002, China.
Abstract:
The development of novel SERS-active MAPbCl3 nanofibers substrate offers a rapid, sensitive, and label-free method for critical stress phytohormone abscisic acid (ABA) detection as compared to conventional methods. Aptamers can act as a specific molecular recognition element that bring ABA molecules closer to the SERS-active MAPbCl3 nanofiber surface, leading to a stronger localized electromagnetic field enhancement. The stable cross dimerized self-complementary (CDSC) aptamer configuration has the lowest Gibbs free energy (ΔG) of -9.75 kcal/mol according to thermodynamics. Bioinformatic analysis of aptamer using OligoAnalyzer® tool offered the thermodynamic properties and functional stability of the aptamer sequence designed to target the LOC109791758 gene encoding the Glycine-Rich Cell Wall Protein (GRCWP) in Cajanus cajan. This facilitated to develop the novel SERS-active single crystal MAPbCl3 nanofiber-based Self-CoAptaNano (SCAN) substrate by improving the target molecule binding and sensitivity even at low concentrations. Material's structure and properties were characterized by using SEM, UV-Visible, and SERS in this study. The currently developed MAPbCl3 nanofiber-based SCAN substrate for ABA detection resulted in better LOD of 1.17 × 10-12 M for SERS and 2.14 × 10-9 M for FLI as compared to previously developed substrates. Moreover, the EF was recorded as 1.08 × 107 M with the recovery rate close to 100 % and RSD of 3.24 % under SERS and 4.13 % under fluorescence exposure in complex matrices for ABA in real plant samples. The adaptability of MAPbCl3 nanofiber-based SCAN as a substrate for aptamer-specific analysis via SERS can underscore their immense potential for broader applications in analytical chemistry and biotechnology.
