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Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
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Using endoplasmic reticulum engineering to improve recombinant protein production in CHO cells

Junhe Zhang1, Weifeng Wang1, Xi Zhang2

  • 1Institutes of Health Central Plains, Xinxiang Key Laboratory for Tumor Drug Screening and Targeted Therapy, Xinxiang Medical University, Xinxiang 453003, China; International Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang 453003, China.

International Journal of Biological Macromolecules
|May 27, 2025
PubMed
Summary

Chinese hamster ovary (CHO) cells are key for producing therapeutic proteins. This review explores endoplasmic reticulum (ER) engineering strategies to overcome ER stress and improve protein secretion, maximizing production yields.

Keywords:
Chinese hamster ovary cellsEndoplasmic reticulum engineeringEndoplasmic reticulum stressRecombinant protein

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