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Restricted effector cell function in ADCC.

P R McConnachie, F Lower, J A McIntyre

    Diagnostic Immunology
    |January 1, 1985
    PubMed
    Summary
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    Investigating antibody-dependent cell-mediated cytotoxicity (ADCC) revealed significant variability in human effector cell function. These differences are not explained by HLA or self-reactivity, suggesting a complex allorestrictive Fc receptor recognition step.

    Area of Science:

    • Immunology
    • Cellular immunology

    Background:

    • Antibody-dependent cell-mediated cytotoxicity (ADCC) is a key immune mechanism.
    • Observed discrepancies in ADCC reactivity among different effector cell populations necessitate further investigation.

    Purpose of the Study:

    • To investigate the variability of human effector cell function in ADCC reactions.
    • To identify factors contributing to differential ADCC reactivity.

    Main Methods:

    • Testing effector cells from ten blood donors against nine antibody-target cell combinations.
    • Utilizing an 11x9 matrix for comprehensive reactivity assessment.
    • Employing exploratory principal components factor analysis.

    Main Results:

    • Identified individuals with weak, intermediate, and strong effector cell functions.

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  • Demonstrated a wide range of ADCC reactivity for each donor's cells across targets.
  • Found no consistent correlation with age, sex, HLA antigen sharing, or self-ADCC.
  • Noted a trend associating strong/weak reactions with HLA-DR7/DR1.
  • Pooled effector cell responses were weaker than anticipated.

    • Conclusions:

    • Human effector cell function in ADCC exhibits significant inter-individual variability.
    • ADCC variability is not explained by simple factors like HLA or self-reactivity.
    • Data suggest an allorestrictive Fc receptor recognition step is crucial in ADCC responses.