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Related Experiment Videos

Reversibly contractile nuclear matrix. Its isolation, structure, and composition.

F Wunderlich, G Herlan

    The Journal of Cell Biology
    |May 1, 1977
    PubMed
    Summary
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    A Tetrahymena nuclear matrix reversibly contracts with changes in calcium and magnesium ion concentrations. This nuclear skeleton

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • The nuclear matrix, a structural component of the nucleus, plays a role in nuclear organization and function.
    • Understanding the dynamic properties of the nuclear matrix can provide insights into nuclear mechanics and processes.

    Purpose of the Study:

    • To isolate and characterize a reversibly contractile nuclear matrix from Tetrahymena macronuclei.
    • To investigate the ionic conditions and molecular components involved in the matrix's contraction.

    Main Methods:

    • Isolation of the nuclear matrix using Triton X-100, DNase, RNase, and high salt extraction.
    • Analysis of matrix composition through biochemical assays and gas chromatography.
    • Electron microscopy to visualize structural components.

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  • SDS-PAGE to identify proteins involved in contraction.

    • Main Results:

    • A reversibly contractile nuclear matrix was isolated, exhibiting a 55% volume reduction under specific cation concentrations (5 mM or 125 mM Ca/Mg).
    • Contraction is independent of ATP and unaffected by salygran, suggesting a non-actin-myosin mechanism.
    • The matrix is primarily protein (96.2%) with residual nucleic acids, phospholipids, and carbohydrates.
    • A predominant 18,000 mol wt protein was identified as potentially involved in the contractile process.

    Conclusions:

    • Tetrahymena nuclear matrix exhibits cation-dependent reversible contraction.
    • The contractile mechanism differs from known biological contractile systems.
    • Further research is needed to elucidate the precise molecular mechanism of this novel nuclear matrix contraction.