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A Standardised Method to Quantify the Infectious Titre of Rabbit Haemorrhagic Disease Virus.

Tiffany W O'Connor¹, Damian Collins¹, Andrew J Read¹

¹Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries and Regional Development, Menangle, NSW 2568, Australia.

|May 28, 2025

Summary

Accurately quantifying rabbit haemorrhagic disease virus (RHDV) infectious titre is crucial for research. This study standardizes the RHDV infectious dose 50% (RID50) estimation method and compares statistical approaches for precise results.

Keywords:

RHDV bioassay virus infectivity virus titration

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Area of Science:

Virology
Veterinary Medicine
Biostatistics

Background:

Quantifying the infectious titre of rabbit haemorrhagic disease virus (RHDV) is vital for research.
Current methods for estimating the RHDV infectious dose 50% (RID50) lack standardization.

Purpose of the Study:

To present a standardized method for estimating RHDV RID50.
To compare statistical methods for RID50 calculation and assess result precision.

Main Methods:

Challenging rabbits with 10-fold serial dilutions of RHDV preparations.
Comparing Reed-Muench, Dragstedt-Behrens, Spearman-Kärber, and probit regression for RID50 calculation.
Calculating 95% confidence intervals using improved Spearman-Kärber and probit regression methods.

Main Results:

All statistical approaches showed high agreement in RID50 calculation.
Improved Spearman-Kärber and probit regression methods provide 95% confidence intervals for precision assessment.

Conclusions:

The standardized method improves the accuracy of RHDV infectious titre estimation.
Accurate RID50 determination is essential for pathogenicity, host resistance, and vaccine studies.