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The COPII Transport Complex Participates in HPV16 Infection.

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|May 28, 2025
PubMed
Summary

Human papillomavirus (HPV) 16 utilizes the endoplasmic reticulum (ER) and COPII pathway for post-Golgi transport. This trafficking is crucial for HPV infection, with Sec24b interacting with viral capsid proteins.

Keywords:
COPIIERERESGolgiHPVmitosispapillomaviruspseudovirustrafficking

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Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Human papillomavirus (HPV) 16 follows a retrograde transport pathway from the cell surface to the Golgi.
  • Post-Golgi trafficking steps, particularly before mitosis, remain poorly understood.
  • Previous studies indicated viral transport to mitotic chromosomes via vesicles.

Purpose of the Study:

  • To elucidate the role of the endoplasmic reticulum (ER) in post-Golgi HPV trafficking.
  • To investigate the significance of the ER-generated coat protein complex II (COPII) anterograde pathway in HPV infection.

Main Methods:

  • Investigated HPV pseudovirus (PsV) colocalization with COPII components.
  • Silenced the COPII pathway to assess its impact on HPV infection.
  • Biochemically isolated and identified interacting proteins between HPV capsid and COPII components.

Main Results:

  • HPV pseudovirus (PsV) demonstrated colocalization with components of the COPII pathway.
  • Inhibition of the COPII pathway significantly reduced HPV infection rates.
  • The inner COPII coat protein, Sec24b, was found to associate with HPV capsid proteins.

Conclusions:

  • The endoplasmic reticulum (ER) plays a critical role in the post-Golgi trafficking of HPV.
  • The COPII anterograde trafficking pathway is essential for efficient HPV infection.
  • Specific COPII components, like Sec24b, directly interact with HPV during its lifecycle.