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Molecular characterization of Serrasalmidae hybrid in the upper Paraná River floodplain using molecular markers
Laura Ivana Ramos1, Eloisa de Páris Paz1, Lidiany Doreto Cavalcanti1
1Programa de Pós-graduação em Ecologia de Ambientes Aquáticos Continentais, Centro de Ciências Biológicas, Universidade Estadual de Maringá, Maringá, Brazil.
Abstract:
This study reports the molecular identification of a tambacu-a hybrid of Colossoma macropomum and Piaractus mesopotamicus-in the floodplain of the upper Paraná River using molecular markers. The PCR-GEL method, based on the analysis of COI and TROP fragments, successfully characterized the hybrid, while PCR-SEQ, employing RAG2 and TROP markers, identified variable sites that differentiate the parental species and the hybrid.
