Related Experiment Video

Updated: Apr 21, 2026

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
07:47

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

12.2K

PqsR-specific quorum sensing inhibitors targeting Pseudomonas aeruginosa: current advances and future directions

Mandsaurwala Aziz1,2, Kafil Ahmed3, Vinothkannan Ravichandran1,4

  • 1Amity Institute of Biotechnology, Amity University Maharashtra, Panvel, Maharashtra, India.

Journal of Chemotherapy (Florence, Italy)
|May 28, 2025
PubMed

Insights

Targeting quorum sensing (QS) in multidrug-resistant Pseudomonas aeruginosa offers a novel strategy. Inhibiting the PqsR regulator disrupts virulence and biofilm formation, enhancing antibiotic effectiveness against resistant bacterial infections.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Drug Resistance

Background:

  • Multidrug-resistant (MDR) Pseudomonas aeruginosa poses a significant threat.
  • Quorum sensing (QS) and biofilm formation are key resistance mechanisms.
  • QS regulates virulence, antibiotic tolerance, and biofilm development.

Purpose of the Study:

  • To review the PqsR-regulated network in P. aeruginosa.
  • To highlight PqsR inhibitors as a strategy to reduce pathogenicity.
  • To explore QS disruption as a method to enhance antibiotic efficacy.

Main Methods:

  • Literature review of PqsR-regulated genes and pathways.
  • Analysis of QS mechanisms in P. aeruginosa.
  • Evaluation of PqsR inhibitors' potential.

Main Results:

  • PqsR is a central regulator of virulence and biofilm genes.
  • Disrupting QS can reduce P. aeruginosa pathogenicity.
  • QS inhibition enhances antibiotic tolerance and efficacy.

Conclusions:

  • PqsR inhibitors represent a promising therapeutic strategy.
  • Targeting QS offers an alternative to direct antibiotic action.
  • Combinatorial approaches targeting QS are effective against MDR P. aeruginosa.
Keywords:
PqsRPseudomonas aeruginosaQuorum sensingmultidrug resistancequorum sensing inhibitors

More Related Videos

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds07:13

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Published on: August 8, 2025

2.0K
Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms03:29

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

1.1K

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
937
Bacterial Signaling01:30

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
43.7K
Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These...
97
Inhibitors of Bacterial Protein Synthesis01:25

Inhibitors of Bacterial Protein Synthesis

Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
82
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
131
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
47