An Off-the-Shelf Artificial Blood Clot Hydrogel Neutralizing Multiple Proinflammatory Mediators for Pro-Regenerative
Yini Huangfu1, Zhezhe Zhao2, Xiang Liu1
1Department of Polymer Science and Engineering, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, P. R. China.
Summary
This study introduces an artificial blood clot hydrogel with platelet-rich plasma (PRP) to combat periodontitis inflammation. The hydrogel effectively reduces inflammatory mediators and promotes periodontal tissue repair.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Immunology
Background:
- Periodontitis is a chronic inflammatory disease characterized by destructive inflammatory mediators.
- Current therapies focus on bacterial elimination, but managing periodontal inflammation remains challenging for regeneration.
- Addressing the inflammatory microenvironment is crucial for effective periodontitis treatment.
Purpose of the Study:
- To develop an artificial blood clot hydrogel encapsulated with platelet-rich plasma (PRP) to mitigate periodontitis-induced inflammation.
- To investigate the immunomodulatory and regenerative capabilities of the engineered hydrogel.
- To evaluate the therapeutic potential of the hydrogel in a rat periodontitis model.
Main Methods:
- Fabrication of a hierarchical fibers-interwoven hydrogel (CCS-RSF@PRP) mimicking a native blood clot.
- Encapsulation of platelet-rich plasma (PRP) within the hydrogel structure.
- In vitro assessment of ROS scavenging, endotoxin neutralization, and cytokine modulation.
- In vivo evaluation in a rat periodontitis model to assess periodontal repair and immune environment normalization.
Main Results:
- The CCS-RSF@PRP hydrogel effectively scavenges reactive oxygen species (ROS) and neutralizes lipopolysaccharide (LPS) and pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β).
- The hydrogel inhibits M1 macrophage polarization and promotes M2 polarization, thereby blocking the chronic inflammatory feedback loop.
- In a rat model, CCS-RSF@PRP hydrogel significantly accelerated periodontium repair by normalizing the periodontal immune environment.
Conclusions:
- The engineered PRP-derived hydrogel effectively mitigates the inflammatory environment in periodontitis.
- The hydrogel mimics and enhances the functions of a natural blood clot, offering a promising therapeutic strategy.
- Local immunomodulation via engineered hydrogels holds significant potential for treating chronic inflammatory diseases like periodontitis.
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