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Quantifying Flavin mononucleotide: an internationally validated methodological approach for enhanced decision making
Keyue Sun1, Chunbao Jiao1, Rebecca Panconesi1
1Department of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.
Ebiomedicine
|May 28, 2025
Summary
Quantifying Flavin mononucleotide (FMN) in donor plasma offers a new way to assess organ viability. This method strongly correlates with liver transplant success and improves organ selection.
Area of Science:
- Transplantation immunology
- Organ preservation
- Biomarker discovery
Background:
- Increasing donor risk necessitates improved organ viability assessment in liver transplantation.
- Current viability parameters lack correlation with post-transplant outcomes.
- Marginal organs require dynamic assessment to enhance utilization.
Purpose of the Study:
- To present a protocol for spectrophotometric quantification of Flavin mononucleotide (FMN).
- To establish FMN as a marker of mitochondrial injury and organ viability.
- To evaluate FMN's predictive value for liver transplant outcomes.
Main Methods:
- Spectrophotometric quantification of FMN in donor plasma and machine perfusion fluids.
- Detailed protocol description including confounders and challenges.
- Analysis of FMN release as an indicator of ischemia-reperfusion injury.
Main Results:
- FMN quantification serves as an immediate organ assessment tool.
- FMN levels strongly correlate with graft survival post-liver transplantation.
- Demonstrated correlation with other relevant post-transplant complications.
Conclusions:
- FMN quantification is a reliable and standardized method for assessing organ viability.
- This method can significantly improve organ selection and utilization.
- FMN offers superior predictive value for transplant outcomes compared to existing parameters.
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