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Predictive Value of Abnormally Expressed Serum MiR-204-5p in Pregnancy during Second Trimester on Subclinical
Qian Wang1, Jingsong Cheng2, Wenjing He3
1Thyroid and Breast Surgery, The Eighth Hospital of Wuhan.
MiR-204-5p is involved in a variety of diseases related to thyroid dysfunction and pregnancy. However, the effect on subclinical hypothyroidism (SCH) during pregnancy remained unclear. We focused on the expression difference of serum miR-204-5p in pregnant women with subclinical hypothyroidism in the second trimester and evaluated the correlation between them in this study. One hundred normal pregnant women and 175 individuals diagnosed with SCH were enrolled. The miR-204-5p level was calculated by qRT-PCR and its relevance to serum thyroid stimulating hormone (TSH) was assessed by Pearson analysis. The predictive value was evaluated by receiver operator characteristic (ROC) curve. The risk factor of SCH was identified by multivariate logistic regression analysis. The miR-204-5p was obviously depressed in SCH group compared to control (P < 0.001) and was significantly negatively correlated to the serum TSH levels (r = -0.6082, P < 0.0001). ROC curve showed the potential predictive value while the area under the curve was 0.9153 with sensitivity of 90.29% and specificity of 86.00%. TSH (OR: 1.793, 95% CI: 1.072-2.998, P = 0.026) and miR-204-5p (OR: 0.528, 95% CI: 0.317-0.878, P = 0.014) were related to SCH risk in pregnant women. In both SCH group and low miR-204-5p group, individuals with respiratory distress syndrome (RDS) or depression possessed a higher proportion of adverse events during 32-38 gestational weeks. Depressed miR-204-5p was observed in SCH patients and was significantly negatively correlated to TSH level. MiR-204-5p was a risk factor for SCH in pregnant women. Additionally, depressed miR-204-5p might be related to the incidence of adverse pregnancy outcomes.
MiR-204-5p is involved in a variety of diseases related to thyroid dysfunction and pregnancy. However, the effect on subclinical hypothyroidism (SCH) during pregnancy remained unclear. We focused on the expression difference of serum miR-204-5p in pregnant women with subclinical hypothyroidism in the second trimester and evaluated the correlation between them in this study. One hundred normal pregnant women and 175 individuals diagnosed with SCH were enrolled. The miR-204-5p level was calculated by qRT-PCR and its relevance to serum thyroid stimulating hormone (TSH) was assessed by Pearson analysis. The predictive value was evaluated by receiver operator characteristic (ROC) curve. The risk factor of SCH was identified by multivariate logistic regression analysis. The miR-204-5p was obviously depressed in SCH group compared to control (P < 0.001) and was significantly negatively correlated to the serum TSH levels (r = -0.6082, P < 0.0001). ROC curve showed the potential predictive value while the area under the curve was 0.9153 with sensitivity of 90.29% and specificity of 86.00%. TSH (OR: 1.793, 95% CI: 1.072-2.998, P = 0.026) and miR-204-5p (OR: 0.528, 95% CI: 0.317-0.878, P = 0.014) were related to SCH risk in pregnant women. In both SCH group and low miR-204-5p group, individuals with respiratory distress syndrome (RDS) or depression possessed a higher proportion of adverse events during 32-38 gestational weeks. Depressed miR-204-5p was observed in SCH patients and was significantly negatively correlated to TSH level. MiR-204-5p was a risk factor for SCH in pregnant women. Additionally, depressed miR-204-5p might be related to the incidence of adverse pregnancy outcomes.
