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Updated: Jan 18, 2026
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Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
Published on: April 20, 2018
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Cadherin-6 controls neuronal migration during mouse neocortical development via an integrin-mediated pathway
Yuki Hirota1, Rikaho Saito1, Takao Honda1,2
1Department of Anatomy, Keio University School of Medicine, Tokyo, Japan.
The FEBS Journal
|May 28, 2025
Summary
Cadherin-6 (CDH6) is crucial for guiding neuronal migration during neocortical development. This study shows CDH6 regulates neuron movement by activating integrin β1, essential for proper brain formation.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Neuronal migration is vital for neocortical development, involving complex signaling pathways and cell adhesion molecules.
- Cadherin-6 (CDH6), a unique cadherin with an RGD integrin-binding motif, has known functions in the nervous system, but its role in neocortical neuronal migration is unexplored.
Purpose of the Study:
- To investigate the role of Cadherin-6 (CDH6) in regulating neuronal migration and positioning during neocortical development.
- To elucidate the molecular mechanisms by which CDH6 influences neuronal movement.
Main Methods:
- Utilized in utero electroporation for Cdh6 knockdown (KD) in the developing cerebral cortex.
- Performed time-lapse imaging to analyze neuronal migration and motility.
- Investigated the interaction between CDH6 and integrin β1 activation.
Main Results:
- CDH6 inhibition led to impaired radial migration and abnormal positioning of cortical neurons.
- CDH6 was found to be essential for maintaining proper neuronal motility.
- CDH6 promotes the activation of integrin β1 on migrating neurons, and this interaction is critical for its function.
Conclusions:
- Cadherin-6 (CDH6) is required for radial migration of cortical excitatory neurons.
- CDH6 regulates neuronal migration by controlling integrin-mediated cell motility.
- The RGD motif in CDH6 is essential for its role in neuronal migration.
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