Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

6.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

17.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.0K
RNA Interference01:23

RNA Interference

26.5K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.5K
MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Formulation of Peptide-Based Nanoparticles Using a Microfluidic Device.

Journal of peptide science : an official publication of the European Peptide Society·2026
Same author

Different contributions of YAP1 and TAZ in the regulation of GIST tumorigenic properties.

Cell communication and signaling : CCS·2026
Same author

International Forum on Visceral Myopathy 2024: Advances in the Knowledge of the Disease.

Neurogastroenterology and motility·2026
Same author

Three-dimensional-mapping of smooth muscle morphogenesis in the vertebrate gastrointestinal tract.

Scientific reports·2025
Same author

Effect of Anorexia Nervosa on Volumetric Bone Mineral Density.

Calcified tissue international·2025
Same author

Sequence engineering at non-motif modulator residues yields a peptide that effectively targets a single PDZ protein in a disease-relevant cellular context.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Sep 20, 2025

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
11:53

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Published on: July 21, 2017

7.4K

Multiprotein Silencing Using WRAP-Based Nanoparticles: A Proof of Concept.

Karidia Konate1, Irène Pezzati1, Karima Redjatti2

  • 1PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214371, Avenue du Doyen G. Giraud, CHU Arnaud de Villeneuve, Bâtiment Crastes de Paulet, 34295 Montpellier, Cedesx 5, France.

Bioconjugate Chemistry
|May 29, 2025
PubMed
Summary
This summary is machine-generated.

This study developed a novel siRNA delivery system using WRAP5 nanoparticles to target multiple cancer-driving genes. This approach effectively reduced glioblastoma and gastrointestinal stromal tumor cell proliferation, offering a promising strategy for personalized cancer therapy.

More Related Videos

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.6K
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

7.8K

Related Experiment Videos

Last Updated: Sep 20, 2025

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
11:53

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Published on: July 21, 2017

7.4K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.6K
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

7.8K

Area of Science:

  • Oncology
  • Molecular Biology
  • Nanotechnology

Background:

  • Cancer remains a leading cause of death, with current treatments facing limitations like side effects and drug resistance.
  • Small interfering RNA (siRNA) offers targeted gene silencing for cancer therapy, but efficacy is limited by single-target approaches.
  • Cell-penetrating peptides (CPPs) integrated into nonviral delivery systems enhance siRNA efficacy.

Purpose of the Study:

  • To enhance therapeutic efficacy by developing a multi-target siRNA delivery system.
  • To investigate the effectiveness of a novel WRAP5 nanoparticle-based delivery system for multiple siRNAs.
  • To evaluate the potential of this approach in glioblastoma and gastrointestinal stromal tumors (GIST).

Main Methods:

  • Designed WRAP5 nanoparticles encapsulating a cocktail of siRNAs targeting CDK4, cyclin D1 (CD1), and MCL-1.
  • Delivered the siRNA cocktail using WRAP5 nanoparticles to human U87 glioblastoma cells.
  • Assessed the impact of the multi-target siRNA delivery on cancer cell proliferation and gene silencing.
  • Evaluated the therapeutic potential in a model of gastrointestinal stromal tumors (GIST).

Main Results:

  • The WRAP5 nanoparticle-delivered siRNA cocktail effectively silenced multiple target genes (CDK4, CD1, MCL-1).
  • Significant reduction in glioblastoma cell proliferation was observed.
  • Demonstrated potential therapeutic impact in gastrointestinal stromal tumors (GIST), known for treatment resistance.

Conclusions:

  • Multi-target siRNA delivery via WRAP5 nanoparticles enhances therapeutic efficacy compared to single-target approaches.
  • WRAP5 nanoparticles represent a versatile platform for personalized cancer therapy.
  • Tailored siRNA delivery systems hold promise for more effective treatment of specific cancer types.