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Updated: Jun 4, 2026

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Published on: September 5, 2015

High-Throughput MicroED for Probing Ion Channel Dynamics.

Marc J Gallenito¹, Max Tb Clabbers^1,2, Jieye Lin¹

¹Department of Biological Chemistry, University of California, Los Angeles, CA, 90095, US.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|May 29, 2025

Summary

This summary is machine-generated.

High-throughput MicroED reveals ion channel dynamics. Structural plasticity in the selectivity filter may explain non-selective ion transport in NaK and NaK2CNG channels.

Keywords:

Ion Channels MicroED ion permeation ion selectivity membrane proteins structural biology

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Area of Science:

Structural biology
Biophysics
Molecular biology

Background:

Ion channels are vital for physiological processes, but their high-resolution structural dynamics remain challenging to visualize.
Understanding ion channel structure is key to elucidating ion permeation and selectivity mechanisms.

Purpose of the Study:

To apply high-throughput MicroED to investigate the structural dynamics of NaK and NaK2CNG ion channels.
To capture distinct structural substates and understand ion channel mechanisms at high resolution.

Main Methods:

High-throughput microcrystal electron diffraction (MicroED) was employed for automated data collection and processing.
Analysis of distinct structural substates from numerous microcrystals of NaK and NaK2CNG.

Main Results:

Consistent sodium binding sites were identified in NaK channel structures.
NaK2CNG exhibited increased dynamics, with potassium binding causing selectivity filter dilation.
The conduction state of NaK2CNG was linked to channel gating, suggesting dynamic control over ion passage.

Conclusions:

The plasticity of the selectivity filter may contribute to the non-selectivity of NaK and NaK2CNG channels.
High-throughput MicroED is a powerful technique for studying ion channel permeation dynamics, complementing computational methods.