Differential significance of Seminal Cell-Free DNA Levels, Oxidative Stress, and Sperm Characteristic between Infertile Men with Non-obstructive and obstructive Azoospermia
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View abstract on PubMed
Summary
This summary is machine-generated.Cell-free DNA (cfDNA) levels in semen are linked to oxidative stress and poor sperm quality in infertile men. Measuring cfDNA and oxidative stress markers may help diagnose male infertility severity and guide antioxidant therapy for improved reproductive outcomes.
Area Of Science
- Reproductive biology
- Andrology
- Biochemistry
Background
- Male infertility affects a significant portion of couples seeking fertility treatment.
- Oxidative stress and impaired sperm parameters are recognized contributors to male infertility.
- Cell-free DNA (cfDNA) in seminal plasma is an emerging biomarker.
Purpose Of The Study
- To investigate seminal cfDNA levels in infertile men.
- To explore the relationship between cfDNA, oxidative stress, antioxidant capacity, and lipid peroxidation in seminal plasma.
- To assess the diagnostic potential of cfDNA and oxidative stress markers in male infertility.
Main Methods
- Semen samples were collected from 100 infertile men with various sperm abnormalities and normozoospermic controls.
- cfDNA was extracted and quantified using quantitative polymerase chain reaction (qPCR).
- Seminal plasma markers of oxidant/antioxidant status and lipid peroxidation were measured.
Main Results
- Elevated seminal cfDNA was observed in men with oligoasthenoteratozoospermia compared to controls.
- A significant decrease in cfDNA was noted in the non-obstructive azoospermia group.
- All patient groups exhibited increased oxidative stress markers (e.g., malondialdehyde) and reduced antioxidant capacity compared to controls.
Conclusions
- A direct correlation exists between elevated seminal cfDNA, increased oxidative stress, and impaired sperm parameters in infertile men.
- cfDNA and oxidative stress markers show potential as diagnostic tools for classifying male infertility severity.
- Antioxidant therapy may improve sperm quality and reproductive outcomes by reducing oxidative damage.
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