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Related Concept Videos

IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

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When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
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In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
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The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
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IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

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In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
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UV–Vis Spectroscopy: Beer–Lambert Law01:09

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The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The...
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Infrared (IR) Spectroscopy: Overview

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When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
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  11. Spectroscopic Characterization Of The Photolysis Of Riboflavin (vitamin B2) Via Time-resolved Mass Spectrometry And Irmpd Spectroscopy

Spectroscopic Characterization of the Photolysis of Riboflavin (Vitamin B2) via Time-Resolved Mass Spectrometry and IRMPD Spectroscopy

Sarah A Wilson1, Aljawharah Alsalem1, Giel Berden2

  • 1Department of Chemistry, University of York, York YO10 5DD, U.K.

The Journal of Physical Chemistry. A
|May 29, 2025

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Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
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View abstract on PubMed

Summary
This summary is machine-generated.

Riboflavin (Vitamin B2) photodegradation accelerates in dimer aggregates, revealing solution conditions significantly impact its stability. This study identifies a novel effect in flavin photochemistry, explaining sensitivity to environmental factors.

Area of Science:

  • Photochemistry
  • Biochemistry
  • Spectroscopy

Background:

  • Riboflavin (Vitamin B2) is a vital photoactive molecule used in food and as a photocatalyst.
  • Existing research on riboflavin photodegradation leaves questions about environmental effects and photoproducts.
  • Understanding these factors is crucial for optimizing riboflavin's applications and stability.

Purpose of the Study:

  • To investigate the 365 nm photolysis of riboflavin under basic conditions using advanced spectroscopic techniques.
  • To characterize the influence of aggregation on riboflavin photodegradation pathways and photoproduct formation.
  • To elucidate the role of the chemical environment in riboflavin's photochemical behavior.

Main Methods:

  • Time-resolved mass spectrometry (TRMS) to monitor riboflavin decay and photoproduct formation.

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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
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  • Gas-phase infrared multiple-photon dissociation (IRMPD) spectroscopy at the FELIX free-electron laser facility.
  • Spectroscopic characterization of riboflavin, its dimer, photoproducts (lumichrome, lumiflavin), and intermediates (formylmethylflavin, riboflavin-lumichrome dimer).

    • Main Results:

    • TRMS successfully tracked light-induced riboflavin decay and the emergence of photoproducts and intermediates.
    • First gas-phase IR spectra of deprotonated riboflavin and its dimer were obtained via IRMPD spectroscopy.
    • Photolysis of riboflavin dimers was observed to be enhanced compared to monomers, suggesting aggregation accelerates degradation.

    Conclusions:

    • Riboflavin aggregation significantly accelerates its photodegradation, a novel finding in flavin photochemistry.
    • The observed enhancement is potentially linked to spectral shifts within the aggregated chromophore.
    • This research provides critical new insights into the sensitivity of riboflavin photodegradation to solution conditions.