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Related Experiment Video

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Isolation and Activation of Murine Lymphocytes
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Reference intervals for functional lymphocyte proliferation studies using 3H thymidine uptake in adults.

Rohan Ameratunga1, Danny Lim2, Hilary Longhurst3

  • 1Department of Clinical Immunology, Auckland Hospital, Park Rd, Grafton, 1010 Auckland, New Zealand; Department of Virology and Immunology, Auckland Hospital, Park Rd, Grafton, 1010 Auckland, New Zealand; Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, University of Auckland, New Zealand.

Journal of Immunological Methods
|May 29, 2025
PubMed
Summary

This study establishes reference intervals for 3H thymidine uptake assays, a key test for cellular immunity. These intervals aid in diagnosing immunodeficiencies and guiding treatment decisions for patients.

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Area of Science:

  • Immunology
  • Clinical Diagnostics

Background:

  • Functional cellular immunity tests are crucial in clinical settings.
  • 3H thymidine uptake assays offer high sensitivity for assessing T cell responses.
  • Assessing cellular immunity is vital for diagnosing immunodeficiencies and managing vaccine efficacy.

Purpose of the Study:

  • To establish reference intervals for 3H thymidine uptake assays.
  • To provide benchmarks for evaluating functional cellular immunity in healthy adults.
  • To support diagnostic laboratories in interpreting cellular immunity test results.

Main Methods:

  • Reference intervals were calculated for over 250 healthy adults.
  • Tests included responses to lectins, anti-CD3 (OKT3), and vaccine antigens (Candida, tetanus, diphtheria).
  • Volunteer blood donors' samples served as controls for patient diagnostic tests.

Main Results:

  • Control samples showed uniform response to PHA.
  • Responses to other lectins, OKT3, and antigens were heterogeneous.
  • Variability in toxoid responses correlated with donors' immunization status.

Conclusions:

  • 3H thymidine uptake assays remain valuable due to sensitivity, despite non-radioactive alternatives.
  • Reference intervals aid in diagnosing conditions like Severe Combined Immunodeficiency (SCID).
  • Results inform clinical decisions regarding live attenuated viral vaccines and hematopoietic stem cell transplantation (HSCT).