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Lichong Decoction represses colorectal cancer progression via inhibiting the PINK1/Parkin pathway regulated by Rab27B

Yu Dai1, Fei Li1, Yao Tang1

  • 1Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine, Southern Medical University, Guangzhou, 510315, Guangdong, China.

Journal of Ethnopharmacology
|May 29, 2025
PubMed
Abstract

Insights

Lichong Decoction (LCD) inhibits colorectal cancer growth by reducing Rab27B expression, which dampens the PINK1/Parkin pathway. This herbal formula effectively suppressed tumor size, migration, and invasion in preclinical models.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Lichong Decoction (LCD) is a traditional herbal formula with demonstrated anti-cancer properties.
  • Its precise mechanisms in colorectal cancer (CRC) progression remain incompletely understood.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which LCD inhibits colorectal cancer progression.
  • To investigate the role of Rab27B and the PINK1/Parkin pathway in LCD's anti-cancer effects.

Main Methods:

  • Establishment of a colorectal cancer xenograft mouse model.
  • High-performance liquid chromatography for LCD constituent analysis.
  • Quantitative real-time PCR, western blotting, and immunohistochemistry to assess Rab27B and PINK1/Parkin pathway protein expression.
  • In vitro cell migration and invasion assays.

Main Results:

  • LCD treatment significantly reduced tumor size in vivo.
  • LCD markedly decreased Rab27B expression in tumor tissues and colorectal cancer cells.
  • Reduced Rab27B expression correlated with suppressed PINK1/Parkin signaling and inhibited cell migration and invasion.

Conclusions:

  • Lichong Decoction exerts its anti-colorectal cancer effects by repressing Rab27B.
  • This repression leads to the dampening of the PINK1/Parkin signaling pathway, consequently inhibiting tumor growth, migration, and invasion.
Keywords:
Colorectal cancerLichong decoctionPINK1/Parkin signalling pathwayRab27B

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