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  • Correction to "A NIR-II Photoacoustic/NIR-IIa Fluorescent Probe for Targeted Imaging of Glioma under NIR-II Excitation"

Correction to "A NIR-II Photoacoustic/NIR-IIa Fluorescent Probe for Targeted Imaging of Glioma under NIR-II Excitation"

  • Shuxin Lyu , Siyu Lu , Conghao Gui , Chunyan Guo , Juanjuan Han , Yuling Xiao , Ruiping Zhang , Xuechuan Hong
Journal of medicinal chemistry +

|

May 29, 2025

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Total Internal Reflection Fluorescence Microscopy 01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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