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Positive Correlation between Lipin-1 and Lipin-2 Expressions and Hepatic T1 Values in IUGR Rats
Tao Wang1, MingZhu Deng1, Alpha Kalonda Mutamba1
1Department of Pediatric, Second Xiangya Hospital of Central South University, Changsha, China.
Background:
Intrauterine growth restriction (IUGR) is associated with long-term metabolic disturbances, including obesity. Changes in hepatic lipid metabolism and adipose tissue function, mediated by lipin-1 and lipin-2, may contribute to these outcomes.
Aim:
This study aimed to investigate the correlation between lipin-1 in visceral adipose tissues (VATs) and lipin-2 in the liver. It also examined hepatic T1 values using T1 mapping in IUGR rats.
Objective:
The objective of this study was to explore the metabolic mechanisms linking IUGR and adult obesity by analyzing molecular and imaging markers.
Methods:
Pregnant rats were fed either a low-protein diet (10%) to induce IUGR or a normal-protein diet (21%) as a control. Male offspring underwent conventional magnetic resonance imaging and native T1 mapping using a 3.0 T whole-body MR scanner at days 21, 56, and 84 post-birth. Liver tissues and VATs were collected for analysis. Lipin-1 and lipin-2 expression levels were measured using Western blot and real-time quantitative PCR.
Results:
The IUGR group exhibited significantly higher mRNA and protein expression levels of lipin-1 and lipin-2 compared to the control group at days 21, 56, and 84 after birth. Additionally, the IUGR group demonstrated significantly higher hepatic T1 values than the control group at the corresponding time points. Positive correlations were observed between the protein and mRNA expression levels of lipin-1 and hepatic T1 values. Similarly, the protein and mRNA expression levels of lipin-2 were positively correlated with hepatic T1 values. All results were statistically significant (P<0.05).
Conclusion:
The upregulation of lipin-1 and lipin-2 expressions was found to be linked to elevated hepatic T1 values, potentially contributing to adult obesity in IUGR rats.
