Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Coronary Artery Disease I: Introduction01:30

Coronary Artery Disease I: Introduction

69
Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
69
Atherosclerosis III: Management01:26

Atherosclerosis III: Management

42
Management of atherosclerosis involves an integrated strategy encompassing pharmacological treatment, surgical interventions, lifestyle changes, and nutrition therapy to address the multifactorial nature of the disease.Pharmacological TherapyA cornerstone of atherosclerosis management is the use of pharmacological agents. Statins, such as atorvastatin, are pivotal in inhibiting HMG-CoA reductase, an enzyme that catalyzes an initial step in cholesterol synthesis in the liver. This reduction in...
42
Atherosclerosis I: Introduction01:30

Atherosclerosis I: Introduction

97
Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
97
Inflammation01:38

Inflammation

55.5K
Overview
55.5K
Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

44
Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
44
Coronary Artery Disease IV: Preventive Measures01:26

Coronary Artery Disease IV: Preventive Measures

40
Effective preventive measures for coronary artery disease (CAD) focus on controlling modifiable risk factors, including cholesterol abnormalities and lifestyle changes.Cholesterol ManagementFirst, the Mediterranean diet and the American Heart Association advocate for maintaining low-density lipoprotein (LDL) cholesterol levels below 100 mg/dL, with a more stringent recommendation of below 70 mg/dL for individuals at high risk. LDL cholesterol, often termed "bad cholesterol," can lead to the...
40

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NLRP3 haploinsufficiency unmasks a compensatory NLRP1-NLRP3 interaction that drives accelerated aging in mice.

Science advances·2026
Same author

Metabolic support of trained immune responses in myeloid cells.

eLife·2026
Same author

Activation of HIF2 in Cardiac Vasculature Leads to Arterial Remodeling, Dilation, Thrombosis, and Inflammation, Recapitulating Cardiac Involvement in Kawasaki Disease.

Circulation·2026
Same author

A signal-responsive cooperative transcription factor network determines alveolar macrophage identity.

The Journal of experimental medicine·2026
Same author

HIF-1α and HIF-2α differentially regulate alveolar macrophage maturation and function.

Cell reports·2026
Same author

Mitochondrial complex I activity promotes antigen cross-presentation in dendritic cells.

Science immunology·2026

Related Experiment Video

Updated: Sep 20, 2025

Quantification of Atherosclerosis in Mice
06:59

Quantification of Atherosclerosis in Mice

Published on: June 12, 2019

38.4K

cDC1s Promote Atherosclerosis via Local Immunity and Are Targetable for Therapy.

Miguel Galán1,2, Laura Fernández-Méndez3,4, Vanessa Núñez1

  • 1Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain (M.G., V.N., M.F.-M., P.F.-B., E.M.-R., S.M.-C., E.H.-G., M.R.-T., A.R.-R., C.R.-R., A.B., A.R.R., I.R.-V., D.S.).

Circulation Research
|May 30, 2025
PubMed
Summary

Conventional type 1 dendritic cells (cDC1s) promote atherosclerosis by enhancing T-cell immunity. Targeting cDC1s with dexamethasone-loaded nanoparticles reduces atherosclerotic lesions without impairing antiviral responses, offering a potential therapeutic strategy.

Keywords:
atherosclerosisdendritic cellsimmunotherapymicenanoparticles

More Related Videos

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
09:06

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Published on: February 20, 2019

8.4K
Flow Cytometry Analysis of Immune Cells Within Murine Aortas
15:15

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Published on: July 1, 2011

34.8K

Related Experiment Videos

Last Updated: Sep 20, 2025

Quantification of Atherosclerosis in Mice
06:59

Quantification of Atherosclerosis in Mice

Published on: June 12, 2019

38.4K
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
09:06

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Published on: February 20, 2019

8.4K
Flow Cytometry Analysis of Immune Cells Within Murine Aortas
15:15

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Published on: July 1, 2011

34.8K

Area of Science:

  • Immunology
  • Cardiovascular Research
  • Dendritic Cell Biology

Background:

  • Atherosclerosis involves immune cell accumulation and T helper 1 (Th1) immunity.
  • The precise role of conventional dendritic cells (cDCs) in orchestrating adaptive T-cell responses in atherosclerosis is debated.

Purpose of the Study:

  • To investigate the function of cDCs in atherosclerosis development.
  • To determine if cDC1s drive adaptive T-cell immunity contributing to atherosclerosis.
  • To explore cDC1-targeted immunotherapy for atherosclerosis.

Main Methods:

  • Utilized Ldlr-/- mice on a high-cholesterol diet (HCD).
  • Manipulated cDC populations via FLT3L overexpression and cDC1 depletion (Xcr1Cre-DTA, Irf8Δ32 models).
  • Analyzed T-cell subsets using flow cytometry and single-cell RNA sequencing (scRNA-seq).
  • Developed and tested anti-CLEC9A targeted nanoparticles loaded with dexamethasone for immunotherapy.

Main Results:

  • cDC expansion exacerbated atherosclerosis; cDC1 depletion prevented it.
  • cDC1 deficiency reduced pro-inflammatory CD4+ Th1 and CD8+ IFN-γ+ T cells.
  • STING pathway in cDC1s was crucial for their proatherogenic function.
  • Targeted nanoparticles reduced atherosclerotic lesions and associated T-cell responses without affecting antiviral immunity.

Conclusions:

  • Conventional type 1 dendritic cells (cDC1s) play a proatherogenic role by promoting CD4+ and CD8+ T-cell immunity.
  • Targeting cDC1s with immunosuppressive nanoparticles presents a potential strategy to mitigate atherosclerosis progression.