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Updated: Sep 20, 2025
In Ovo Feeding of Commercial Broiler Eggs: An Accurate and Reproducible Method to Affect Muscle Development and Growth
Published on: September 20, 2021
Using a Multi-Attribute Decision-Making Method to Evaluate the Effect of in Ovo Injection of Valine and Tryptophan on
Marziyeh Ebrahimi1, Hoda Javaheri Barfourooshi2, Seyed Abdullah Hosseini2
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Background:
Eggs provide limited energy and essential nutrients necessary for chicken embryo growth. Intra-egg nutrition serves as an effective approach to address initial growth constraints during the embryonic stage and post-hatching.
Objective:
This study aimed to identify the optimal levels of L-valine or L-tryptophan amino acids administered via in ovo injection to improve the growth performance of Ross 308 broiler chickens.
Methods:
Five hundred fertile eggs were weighed and incubated. On Day 14, the fertile eggs were divided into five groups, receiving injections of either 0.95% (Val-1) or 1.05% (Val-2) L-valine, 0.55% (Trp-1) or 0.60% (Trp-2) L-tryptophan, or distilled water (control) at a volume of 0.5 mL. Nine performance criteria, including hatchability, body weight, feed intake, feed efficiency, livability, production index, villus length to crypt depth, Lactobacillus and Escherichia coli populations were assessed as least-square means.
Results:
A multiple-attribute decision-making (MADM) method, utilising the technique for order of preference similarity to the ideal solution (TOPSIS), was employed to rank the in ovo amino acid treatments. The treatments yielded the following scores: control (0.7703), Val-1 (0.4759), Val-2 (0.4161), Trp-1 (0.7761) and Trp-2(0.1509).
Conclusions:
The findings indicate that injecting 0.55% L-tryptophan on Day 14 of incubation is a beneficial strategy to enhance growth performance in broiler chickens.
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