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TaPHT1;9 Phosphate Transporter Mediates Selenite Absorption in Wheat
Chuang Lou1, Yaxin Wang1, Zehua Fan1
1National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Journal of Agricultural and Food Chemistry
|May 30, 2025
Summary
This study identifies TaPHT1;9 as a key gene in wheat for absorbing selenite, a form of selenium. Understanding this gene can help breed wheat with higher selenium content for improved nutrition.
Area of Science:
- Plant Biology
- Agricultural Science
- Nutritional Science
Background:
- Selenium (Se) is an essential human micronutrient obtained from food.
- Phosphate transporters (PHTs) are known to mediate selenite absorption in plants.
- Specific PHTs involved in wheat selenite uptake remained unidentified.
Purpose of the Study:
- To identify specific wheat phosphate transporters (PHTs) responsible for selenite uptake.
- To investigate the role of identified PHTs in selenium accumulation and plant growth.
- To explore the potential of candidate genes for developing selenium-enriched wheat varieties.
Main Methods:
- Gene expression analysis of wheat phosphate transporters under selenium treatment.
- Functional characterization of candidate genes using yeast complementation assays.
- CRISPR-Cas9 gene editing in wheat to create mutants for selenite uptake studies.
- Ectopic expression of candidate genes in transgenic rice to assess selenium accumulation and growth phenotypes.
- Analysis of selenium content in grains of wheat varieties with different haplotypes.
Main Results:
- Wheat gene TaPHT1;9 was significantly upregulated by selenium treatment and enhanced selenite adsorption in yeast.
- CRISPR-edited wheat mutants lacking functional TaPHT1;9 showed increased biomass but decreased selenium content.
- Transgenic rice overexpressing TaPHT1;9 exhibited reduced growth and higher selenium accumulation.
- Wheat varieties with the elite haplotype Hap3 of TaPHT1;9 had higher grain selenium content.
Conclusions:
- TaPHT1;9 plays a crucial role in mediating selenite uptake in wheat.
- TaPHT1;9 is a promising candidate gene for breeding selenium-enriched wheat varieties.
- Targeting TaPHT1;9 can enhance selenium biofortification in wheat crops.
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