A Facile Coordination Polymer Nanoparticle for Sonodynamic Therapy Combating Drug-Resistant Bacteria

Area of Science:

• Biomaterials Science
• Nanotechnology
• Infectious Diseases

Background:

• Bacterial infections and biofilms pose significant challenges due to drug resistance.
• Effective treatments for severe bacterial infections are urgently needed.

Purpose of the Study:

• To develop a coordination polymer nanoparticle (LZC) for sonodynamic therapy (SDT) against drug-resistant bacteria.
• To enhance the efficacy of SDT in combating biofilms and deep-tissue infections.

Main Methods:

• Fabrication of LZC nanoparticles by conjugating porphyrin chlorin e6 (Ce6) with LL37 via coordination interaction.
• Utilizing ultrasound (US) to stimulate LZC nanoparticles for reactive oxygen species (ROS) and heat generation.
• In vitro and in vivo evaluations of antibacterial activity, biofilm eradication, and therapeutic efficacy in a mouse model.

Main Results:

• LZC nanoparticles exhibited uniform size and positive surface charge, facilitating bacterial capture and biofilm penetration.
• US-triggered LZC nanoparticles generated significantly higher ROS and heat compared to free sonosensitizers.
• Achieved up to 99% eradication of multi-drug resistant Pseudomonas aeruginosa (MDRPA) and significant biofilm disruption.
• Demonstrated therapeutic potential in an MDRPA-induced pneumonia mouse model.

Conclusions:

• LZC coordination polymer nanoparticles represent an innovative strategy to enhance SDT for treating drug-resistant bacterial infections.
• This approach shows great promise for advancing therapeutic interventions in deep tissue bacterial infections.