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Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
Published on: October 31, 2019
Iron-restricted Mycobacterium tuberculosis exports pathogenicity factors packed in extracellular vesicles
Nishant Sharma1, Nevadita Sharma1, Ashis Biswas1
1The Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey, United States of America.
None:
Mycobacterium tuberculosis, the pathogen responsible for human tuberculosis, responds to iron limitation by increasing the production of extracellular vesicles. This study examined the protein composition of induced M. tuberculosis extracellular membrane vesicles using chromatography coupled with mass spectrometry. The results revealed that vesicles contain key pathogenicity factors, including proteins that enhance bacterial survival, immune evasion, and inflammation. These findings deepen our understanding of the potential role of extracellular vesicles in M. tuberculosis-host interactions. The data can also aid in identifying new biomarkers of infection and developing vesicle-based, culture-independent TB diagnostic platforms.
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