Related Experiment Video
Updated: Jun 16, 2025
05:15
An Aptamer-based Sensor for Unchelated GadoliniumIII
Published on: January 9, 2017
7.2K
Glycan-Induced Transchelation of Gadolinium from Magnetic Resonance Imaging Contrast Agent-Complexes
Lukasz Polewski1,2, Daria Dymnikova3, Weronika Malicka1
1Institute of Chemistry and Biochemistry, Freie Universität Berlin, Altensteinstrasse 23a, 14195 Berlin, Germany.
Analytical Chemistry
|May 30, 2025
Summary
This study provides direct evidence that glycosaminoglycans (GAGs) bind gadolinium. This binding mechanism is crucial for understanding gadolinium deposition disease after MRI contrast agent use.
Area of Science:
- Biochemistry
- Biophysics
- Materials Science
Background:
- Glycosaminoglycans (GAGs) are vital extracellular matrix components.
- GAGs may release gadolinium from MRI contrast agents, potentially causing gadolinium deposition disease.
- The molecular mechanisms of gadolinium release by GAGs are not well understood.
Purpose of the Study:
- To provide direct evidence of gadolinium binding by GAGs.
- To elucidate the molecular interactions between GAGs and gadolinium.
- To understand the initial steps in gadolinium release from contrast agents.
Main Methods:
- Utilized synthetic Fondaparinux (FPX), a heparin mimetic, as a model GAG.
- Employed mass spectrometry and electron paramagnetic resonance (EPR) spectroscopy to detect FPX-gadolinium complexes.
- Characterized binding using EPR, isothermal titration calorimetry, and gas-phase infrared (IR) spectroscopy.
- Investigated the transchelation process via collision-induced dissociation experiments.
Main Results:
- Directly observed complexes formed between FPX and gadolinium.
- Quantified the binding affinity and thermodynamics of FPX-gadolinium interactions.
- Provided molecular-level insights into the gadolinium binding and release process.
Conclusions:
- GAGs directly bind gadolinium ions.
- This binding is a key factor in gadolinium release from MRI contrast agents.
- Findings contribute to understanding the etiology of gadolinium deposition disease.
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