Affinity chromatographic examination of a retention model for macromolecules

Plots of log k' vs. log (1/[mobile phase modifier]) were made for a monovalent and a divalent solute using affinity chromatography. Some of the plots were curved and all exhibited slopes (Z values) of less than the theoretical integer values. It was shown that this was an expected result when lower forms of the solute were present, e.g., a divalent solute adsorbed monovalently.