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Related Experiment Video

Updated: Sep 19, 2025

The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis
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The Epigenetic Reader PHF23 Is Required for Embryonic Neurogenesis.

Yue Wen1,2,3, Ping He1, Zongyao Huang1

  • 1Department of Neurosurgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|May 30, 2025
PubMed
Summary
This summary is machine-generated.

PHF23 is essential for embryonic brain development, regulating neurogenesis by interacting with HDAC2. Its absence causes cortical defects due to blocked radial glial cell differentiation.

Keywords:
PHD finger proteinsPHF23epigeneticsneural stem cellneurogenesis

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Area of Science:

  • Neuroscience
  • Epigenetics
  • Developmental Biology

Background:

  • Epigenetic mechanisms are vital for neurogenesis during embryonic development.
  • Plant homeodomain (PHD) finger proteins are epigenetic readers involved in development, but their role in embryonic neurogenesis is unclear.

Purpose of the Study:

  • Investigate the role of PHF23, a PHD finger protein, in embryonic neurogenesis.
  • Determine the molecular mechanisms by which PHF23 regulates neuronal differentiation.

Main Methods:

  • Differential expression analysis of PHD finger proteins during neurogenesis.
  • Utilized mouse models (Phf23 knock-out) to study neurogenesis.
  • Investigated protein-protein interactions (PHF23-HDAC2) and histone modification (H3K27ac).

Main Results:

  • PHF23 is highly expressed in radial glial cells (RGCs) and intermediate progenitor cells (IPCs), but not neurons.
  • Phf23 knock-out mice exhibit cortical developmental defects linked to RGC differentiation blockade.
  • PHF23 inhibits HDAC2 activity, preventing H3K27ac deacetylation and promoting neuronal gene expression (e.g., Tcf4, Eya1).
  • Tcf4 overexpression rescued differentiation defects in Phf23-knockout neural stem cells.

Conclusions:

  • PHF23 is a critical regulator of embryonic neurogenesis and cortical development.
  • PHF23 acts by modulating HDAC2 activity to control histone acetylation and gene expression.
  • These findings highlight cell type-specific functions of PHD finger proteins in neurogenesis.