Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.3K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effective treatment of human breast tumors by chimeric CCL2 and CCL8 diphtheria toxin cytotoxic peptides.

Cancer biology & therapy·2026
Same author

Multiple mTOR RNA localization signals regulate subcellular protein synthesis and axonal growth.

bioRxiv : the preprint server for biology·2026
Same author

A mouse model of autosomal dominant spastic ataxia and myopathy caused by a mutation in <i>Tuba4a</i>.

bioRxiv : the preprint server for biology·2026
Same author

Evaluating methylphenidate as a potential treatment for cocaine use disorder: a propensity score-matched study.

The American journal of drug and alcohol abuse·2026
Same author

Evaluating the Impact of Blood-Brain Barrier-Penetrant ACE Inhibitors on HIV-Associated Neurocognitive Disorders.

Journal of acquired immune deficiency syndromes (1999)·2026
Same author

Evaluation of Nicotinamide Riboside in Prevention of Small Nerve Fiber Axon Degeneration and Promotion of Nerve Regeneration.

Journal of the peripheral nervous system : JPNS·2026

Related Experiment Video

Updated: Jun 14, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.2K

Single-particle tracking of genetically encoded nanoparticles: Optimizing expression for cytoplasmic diffusion

Elizaveta Korunova1, Vitali Sikirzhytski1, Jeffery L Twiss2

  • 1Department of Drug Discovery & Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, South Carolina.

Biophysical Journal
|May 31, 2025
PubMed
Summary

We optimized genetically encoded multimerics (GEMs) for tracking cytoplasmic diffusion in mammalian cells. A new inducible system controls GEM particle density, improving imaging and analysis for studying cellular environments.

More Related Videos

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

2.2K
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

8.1K

Related Experiment Videos

Last Updated: Jun 14, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.2K
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

2.2K
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

8.1K

Area of Science:

  • Cell biology
  • Biophysics
  • Nanotechnology

Background:

  • Single-particle tracking (SPT) probes cytoplasmic properties using fluorescent nanoparticles.
  • Genetically encoded multimerics (GEMs) are 40-nm particles suitable for studying diffusion similar to ribosomes and protein complexes.
  • Understanding GEM expression effects on diffusivity is crucial for accurate cytoplasmic analysis.

Purpose of the Study:

  • To optimize GEM tracking in mammalian cells by controlling expression levels.
  • To assess the impact of GEM expression density on measured cytoplasmic diffusivity.
  • To enhance GEM diffusivity analysis by accounting for motion types and heterogeneity.

Main Methods:

  • Developed and compared a doxycycline-inducible GEM expression system with a constitutive system.
  • Quantified GEM particle density in response to doxycycline concentration and incubation time.
  • Incorporated an effective diffusion coefficient and standard deviation of displacements for advanced analysis.

Main Results:

  • The inducible GEM system significantly reduced particle density (5-500 per area) compared to constitutive systems (up to 2000).
  • Optimized particle density improved imaging and cellular homogeneity.
  • Enhanced analysis methods provided a more nuanced understanding of GEM diffusivity.

Conclusions:

  • A doxycycline-inducible GEM system offers precise control over particle density for improved SPT in mammalian cells.
  • Optimized GEM tracking facilitates the study of cytoplasmic physical properties under various conditions.
  • Advanced diffusivity analysis enhances the interpretation of particle motion in complex cellular environments.