Isolation of Extracellular Vesicle Subtypes from Primary Osteoblast Cultures

This chapter describes the isolation and characterization of extracellular vesicles (EVs) isolated from primary rodent osteoblasts and extracellular matrix. The key advantages of this assay are that it allows the isolation of EVs from the conditioned media of primary osteoblasts at varying stages of differentiation and subsequent characterization prior to downstream applications.