Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

92.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.8K
Sanger Sequencing01:57

Sanger Sequencing

758.2K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
758.2K
The DNA Helix01:07

The DNA Helix

25.7K
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
25.7K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.5K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.5K
DNA Isolation01:34

DNA Isolation

194.4K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
194.4K
DNA Microarrays02:34

DNA Microarrays

18.7K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
18.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deciphering the mechanism of action of metal contaminants on forensic DNA Analysis: An in-silico and in-vitro study of STR Interference.

Gene·2026
Same author

Detrimental effect of metal contaminants on forensic DNA analysis and strategies for mitigation.

International journal of legal medicine·2026
Same author

Advanced molecular techniques in distinguishing monozygotic twins for forensic applications.

Genes & genomics·2026
Same author

Genealogically bewildered individuals and forensic identification: a review of current and emerging solutions.

International journal of legal medicine·2025
Same author

Impact of population size on population genetic analysis of Short Tandem Repeat (STR) allelic data, forensic and paternity parameters and its effect on forensic DNA analysis.

Forensic science, medicine, and pathology·2024
Same author

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases.

International journal of legal medicine·2024

Related Experiment Video

Updated: Sep 19, 2025

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

15.9K

DNA forensics at forty: the way forward.

Hirak Ranjan Dash1,2, Noora R Al-Snan3

  • 1School of Forensic Science, National Forensic Sciences University, Delhi Campus, New Delhi, 110085, India. hirakdash@gmail.com.

International Journal of Legal Medicine
|May 31, 2025
PubMed
Summary

Forensic DNA analysis has advanced significantly over 40 years, improving criminal investigations. Future innovations aim to overcome challenges like backlogs and enhance DNA analysis with AI and new technologies.

Keywords:
Artificial intelligence (AI) in forensicsDNA databasesForensic DNA analysisNext-generation sequencing (NGS)Rapid DNA technology

More Related Videos

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

20.9K
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.1K

Related Experiment Videos

Last Updated: Sep 19, 2025

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

15.9K
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

20.9K
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.1K

Area of Science:

  • Forensic Science
  • Genetics
  • Criminal Justice

Background:

  • Forensic DNA analysis, established in 1985, has revolutionized criminal investigations.
  • The field has progressed through distinct developmental phases over four decades.
  • Technological leaps have enhanced speed, accuracy, and application in forensic science.

Purpose of the Study:

  • To review the evolution of forensic DNA analysis over the past 40 years.
  • To identify current challenges and emerging technologies in the field.
  • To outline future directions for forensic DNA analysis.

Main Methods:

  • Review of key advancements in DNA analysis techniques.
  • Discussion of challenges including case backlogs and sample types.
  • Exploration of emerging technologies like AI, ML, and single-cell genomics.

Main Results:

  • Significant improvements in speed and reliability through techniques like rapid DNA analysis and next-generation sequencing.
  • Persistent challenges include case backlogs, limited databases, and analysis of degraded DNA.
  • Emerging technologies and AI/ML integration promise enhanced efficiency and accuracy.

Conclusions:

  • Forensic DNA analysis is a continuously evolving field with substantial progress.
  • Further innovation is needed to address current limitations and ethical considerations.
  • Future efforts will focus on database expansion, quality control, and global training standardization.