Related Experiment Video
Updated: Sep 19, 2025
07:55
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Published on: March 7, 2019
8.2K
Protocol for predicting suppressors of cell-death pathways based on transcriptomic and vulnerability data.
Yaron Vinik1, Avi Maimon1, Sima Lev1
1Molecular Cell Biology Department, Weizmann Institute of Science, Rehovot 76100, Israel.
STAR Protocols
|May 31, 2025
Summary
This study introduces a computational method to predict repressors of cancer cell death. The protocol identifies potential cell death repressors by analyzing gene vulnerability and transcriptomic data, aiding in cancer research.
Area of Science:
- Computational Biology
- Cancer Research
- Genomics
Background:
- Understanding mechanisms that regulate cancer cell death is crucial for developing effective cancer therapies.
- Identifying genes that protect cancer cells from death (repressors) can reveal new therapeutic targets.
Purpose of the Study:
- To present a computational protocol for predicting cancer cell death repressors.
- To combine gene vulnerability and transcriptomic responses to cell death inducers for prediction.
Main Methods:
- Developed a protocol to calculate gene predictors based on vulnerability and transcriptomic data.
- Aggregated predictors into a single metric to rank genes by their predictive power for cell death repression.
- Established criteria for selecting candidate genes for experimental validation.
Main Results:
- The computational protocol successfully predicts potential repressors of cancer cell death.
- Several experimentally validated cell death repressors were identified using the developed protocol.
Conclusions:
- The presented computational protocol is an effective tool for identifying cancer cell death repressors.
- This method facilitates the discovery of novel therapeutic targets in cancer treatment.
More Related Videos
Related Concept Videos
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
8.2K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.2K
01:12Loss of Tumor Suppressor Gene Functions
5.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.1K
01:30Overview of Cell Death
7.8K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.8K