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  11. Bacteriostatic, Silver-doped, Zirconia-based Thin Coatings For Temporary Fixation Devices Tuning Stem Cells' Expression Of Adhesion-relevant Genes And Proteins

Bacteriostatic, silver-doped, zirconia-based thin coatings for temporary fixation devices tuning stem cells' expression of adhesion-relevant genes and proteins

Sara Ferraris1, Alessandro C Scalia2, Mauro Nascimben2

  • 1Department of Applied Science and Technology, Politecnico di Torino, Italy.

Biomaterials Advances
|May 31, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

A novel zirconia-silver coating on temporary fixation devices shows bacteriostatic properties and prevents cell adhesion. This biocompatible coating supports bone healing without interfering with implant removal.

Area of Science:

  • Biomaterials Science
  • Orthopedic Surgery
  • Nanotechnology

Background:

  • Temporary fixation devices are crucial for bone healing, requiring biocompatibility, ease of removal, and infection prevention.
  • Current devices face challenges with bone tissue overgrowth and infection risk.
  • Developing advanced coatings is essential to meet these clinical demands.

Purpose of the Study:

  • To develop and characterize thin zirconia-silver (ZrO2-Ag) coatings on Ti6Al4V for temporary fixation devices.
  • To evaluate the cytocompatibility, anti-adhesion, and bacteriostatic effects of low-silver concentration coatings.
  • To investigate the molecular mechanisms underlying the observed anti-adhesion effects.

Main Methods:

  • Sputtering of ZrO2-Ag coatings with low (0.2 at% Ag - AL) and high (0.5 at% Ag - AH) silver concentrations on Ti6Al4V.
Keywords:
AntiadhesionAntibacterialCoatingsOMICS

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  • Characterization of surface properties: silver content, release kinetics, mechanical adhesion, morphology, roughness, wettability, and zeta potential.
  • In vitro assessment of direct cytocompatibility with human mesenchymal stem cells (hMSC) and antibacterial activity against Staphylococcus aureus, followed by multi-omics analysis (transcriptomics, proteomics) and bioinformatics.

    • Main Results:

    • Coatings exhibited high stability with continuous silver release over 28 days.
    • The AL coating demonstrated direct cytocompatibility with hMSC and significant anti-adhesion effects.
    • Multi-omics analysis revealed down-regulation of genes and proteins involved in cell adhesion (cadherins, integrins, Talin, RhoA) without affecting apoptosis.
    • Bacteriostatic properties were confirmed for the AL coating.

    Conclusions:

    • The low-silver concentration ZrO2-Ag coating (AL) is a promising candidate for temporary bone fixation devices.
    • AL coatings are cytocompatible, support bone healing, prevent implant-to-bone adhesion, and offer bacteriostatic effects.
    • The anti-adhesion mechanism involves modulating cell-substrate interactions at the molecular level.
    Temporary fixation devices