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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Production, optimisation and characterisation of exopolysaccharides from Aureobasidium pullulans Y428 using Melinjo
Indah Widyastuti1, Saleh Wikarsa1, Sundani Nurono Soewandhi1
1Department of Pharmaceutical Science, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia.
Abstract:
Exopolysaccharides (EPS) are multifunctional biopolymers with applications in the pharmaceutical, food, and cosmetic industries due to its bioactivity characteristics. This study screened strains capable of producing EPS Y428, which was isolated from Piper betle stem and identified as Aureobasidium pullulans based on its internally transcribed spacer (ITS) analysis, as well as its characteristics, colony morphology, and structure. Fourier-transform infra-red spectroscopy (FTIR), liquid chromatography-high-resolution mass spectrometry (LC-HRMS), and high-performance liquid chromatography (HPLC) confirmed the presence of a trisaccharide composed of glucose and fructose, molecular weight of 2.3 × 104 Da. Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) established EPS stability at 219.32 °C, while X-ray diffraction (XRD) revealed a semi-crystalline structure, and scanning electron microscopy (SEM) demonstrated a smooth, rigid layered structure. These findings indicate that Y428 has promising potential to support global efforts in industrial innovation.
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