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Recombinant DNA

Recombinant DNA

Cloning of Dolly the Sheep

Cloning of Dolly the Sheep

The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual, the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...

Introduction to Nuclear Reprogramming

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...

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Related Experiment Video

Updated: Sep 19, 2025

CRISPR Guide RNA Cloning for Mammalian Systems

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

Brief guide to gene cloning.

Woojin Hong¹, Seokjun G Ha¹, Hyunwoo C Kwon¹

¹Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 34141, South Korea.

Molecules and Cells

|May 31, 2025

Summary

This summary is machine-generated.

Gene cloning is a key molecular biology technique for isolating and amplifying DNA. This guide covers essential processes, types, and applications for researchers performing DNA analysis.

Keywords:

Cloning Ligation Restriction enzyme Selection Transformation

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Last Updated: Sep 19, 2025

CRISPR Guide RNA Cloning for Mammalian Systems

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

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Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Gene cloning is a fundamental technique for DNA manipulation.
It enables the isolation, amplification, and production of recombinant DNA molecules.
Understanding gene function and expression relies heavily on DNA analysis.

Discussion:

This guide provides an overview of gene cloning processes.
It details various types of gene cloning methods.
Key experimental considerations for efficient DNA analysis are highlighted.

Key Insights:

Gene cloning is essential for molecular biology research.
The technique facilitates the study of gene function and expression.
This resource aids researchers new to gene cloning.

Outlook:

Further advancements in gene cloning techniques are expected.
Applications in synthetic biology and genetic engineering will expand.
Efficient DNA analysis remains critical for biological research.