Codonopsis pilosula polysaccharides attenuate lipid accumulation and inflammatory response in the NAFLD mouse by...

This study investigated the effects of Codonopsis pilosula polysaccharides (CPP) on nonalcoholic fatty liver disease (NAFLD) induced by a high-fat diet in mice and its underlying mechanisms. Mice were divided into six groups: control, model, fenofibrate, and three CPP dosage groups (300, 200, 100 mg/kg). CPP reduced liver lipid accumulation, serum TC, TG, LDL-C, ALT, AST, MDA, IL-6, and TNF-α levels while increasing HDL-C, SOD, and GSH-Px levels (P < 0.05). Histopathological analysis confirmed decreased lipid deposition. Western blot showed CPP enhanced AMPK and ACC phosphorylation, while RT-PCR revealed reduced expression of SREBP1 and its target genes. In PA-induced HepG2 cells, CPP effects were reversed by AMPK inhibition. These findings demonstrate CPP's potential to mitigate hepatocellular lipotoxicity, oxidative stress, and inflammation in NAFLD through the AMPK/ACC/SREBP1 pathway, offering a promising therapeutic approach.