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Atherosclerosis III: Management01:26

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Related Experiment Video

Updated: Sep 19, 2025

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Codonopsis pilosula polysaccharides attenuate lipid accumulation and inflammatory response in the NAFLD mouse by

Qiu Chen1, Hai Lin1, Xianen Huang1

  • 1Department of endocrinology, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325200, China.

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|June 1, 2025
PubMed
Summary

Codonopsis pilosula polysaccharides (CPP) show promise in treating nonalcoholic fatty liver disease (NAFLD). CPP effectively reduced liver fat, inflammation, and oxidative stress in mice by activating the AMPK/ACC/SREBP1 pathway.

Keywords:
AMPK/ACC/SREBP1 signaling pathwayCodonopsis pilosula polysaccharideInflammatory factorsNonalcoholic fatty liverdiseaseOxidative stress

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Area of Science:

  • Pharmacology
  • Hepatology
  • Biochemistry

Background:

  • Nonalcoholic fatty liver disease (NAFLD) is a prevalent condition characterized by excessive fat accumulation in the liver.
  • High-fat diets are a primary driver of NAFLD pathogenesis, leading to hepatocellular damage and inflammation.
  • Current therapeutic strategies for NAFLD are limited, necessitating the exploration of novel treatments.

Purpose of the Study:

  • To investigate the therapeutic effects of Codonopsis pilosula polysaccharides (CPP) on high-fat diet-induced nonalcoholic fatty liver disease (NAFLD) in a mouse model.
  • To elucidate the underlying molecular mechanisms by which CPP exerts its protective effects against NAFLD.
  • To evaluate CPP's impact on lipid metabolism, oxidative stress, and inflammatory markers in NAFLD.

Main Methods:

  • Establishment of a high-fat diet-induced NAFLD mouse model.
  • Administration of varying dosages of CPP (100, 200, 300 mg/kg) and fenofibrate as a positive control.
  • Biochemical assays to measure serum lipid profiles, liver enzymes, oxidative stress markers, and inflammatory cytokines.
  • Histopathological examination of liver tissues for lipid deposition.
  • Western blot and RT-PCR analyses to assess key protein and gene expression involved in lipid metabolism and signaling pathways (AMPK, ACC, SREBP1).
  • In vitro studies using PA-induced HepG2 cells to confirm the role of the AMPK pathway.

Main Results:

  • CPP treatment significantly reduced liver lipid accumulation and improved NAFLD histological features.
  • CPP administration led to a significant decrease in serum levels of total cholesterol (TC), triglycerides (TG), and LDL-C, while increasing HDL-C.
  • CPP effectively lowered markers of liver injury (ALT, AST), oxidative stress (MDA), and inflammation (IL-6, TNF-α), and enhanced antioxidant enzymes (SOD, GSH-Px).
  • Molecular analysis revealed that CPP promotes the phosphorylation of AMPK and ACC, and reduces the expression of SREBP1 and its downstream targets.
  • In vitro experiments confirmed that CPP's effects are mediated through the AMPK pathway, as inhibition of AMPK reversed the protective effects.

Conclusions:

  • Codonopsis pilosula polysaccharides (CPP) demonstrate significant efficacy in ameliorating NAFLD in a mouse model.
  • CPP exerts its therapeutic benefits by mitigating hepatocellular lipotoxicity, oxidative stress, and inflammation.
  • The underlying mechanism involves the activation of the AMPK/ACC signaling pathway, leading to the downregulation of SREBP1-mediated lipogenesis.