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Production of Organic Acids01:25

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Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

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Lignin Fractionation Coupling Laccase-Mediated Depolymerization Enhancing Biological Lignin Valorization.

Rongqian Meng1,2,3, Siying Zhang3, Yihan Zhang3

  • 1Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Journal of Agricultural and Food Chemistry
|June 2, 2025
PubMed
Summary

Fractionating lignin into smaller pieces and using laccase enzymes significantly improves its conversion into valuable products like polyhydroxyalkanoates (PHA) by microbes.

Keywords:
bioconversionbiorefinerylaccaselignin fractionationlignin valorization

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Area of Science:

  • Biotechnology
  • Biorefining
  • Microbial Engineering

Background:

  • Lignin bioconversion is key for sustainable biorefining, but its complex structure hinders efficiency.
  • Microbial conversion of lignin into value-added chemicals and biofuels faces challenges due to lignin's inherent heterogeneity.

Purpose of the Study:

  • To investigate how lignin fractionation impacts bioconversion performance using *Pseudomonas putida* KT2440.
  • To explore the combined effects of lignin fractionation and enzymatic treatment (laccase-HBT) on microbial lignin valorization.

Main Methods:

  • Lignin fractionation to obtain different molecular weight fractions (F0, F1, F2, F3).
  • Fermentation of *Pseudomonas putida* KT2440 on various lignin fractions.
  • Enzymatic treatment using laccase and 1-hydroxybenzotriazole hydrate (HBT).
  • Analysis of polyhydroxyalkanoate (PHA) yield and bacterial growth.
  • Heteronuclear single quantum correlation (HSQC) analysis to assess lignin structure changes.

Main Results:

  • The lowest molecular weight lignin fraction (F3) yielded the highest cell amounts, PHA yield (0.35 g/L), and lignin degradation.
  • Laccase-HBT treatment significantly increased viable bacteria across all fractions.
  • The highest PHA yield (0.41 g/L) was achieved with F3 treated with laccase-HBT, outperforming untreated and laccase-treated samples.
  • Laccase-HBT effectively cleaved β-O-4 bonds, leading to a more uniform low molecular weight lignin.

Conclusions:

  • Lignin fractionation is crucial for enhancing microbial bioconversion efficiency.
  • Combining lignin fractionation with laccase-HBT catalysis optimizes lignin valorization into PHA.
  • This integrated approach facilitates microbial lignin valorization by producing a uniform, low molecular weight lignin substrate.