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OMIP-115: High-Dimensional Phenotypic Characterization of Human Natural Killer Cells for Therapeutic Use

  • 0Centre for Cellular Immunotherapy of Haematological Cancer Odense (CITCO), Department of Clinical Immunology, Odense University Hospital, University of Southern Denmark, Odense, Denmark.
Cytometry. Part a : the Journal of the International Society for Analytical Cytology +

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June 2, 2025

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June 2, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

A new 29-color flow cytometry panel offers detailed characterization of human peripheral blood Natural Killer (NK) cells. This tool aids in preclinical development and monitoring of NK cell therapies by assessing differentiation, cytotoxicity, and dysfunction.

Area Of Science

  • Immunology
  • Cell Biology
  • Biotechnology

Background

  • Natural Killer (NK) cells are crucial for innate immunity and cancer immunotherapy.
  • Standardized methods for characterizing therapeutic NK cells are essential for clinical translation.
  • Existing methods may not capture the full spectrum of NK cell phenotypes and functions.

Purpose Of The Study

  • To develop and optimize a comprehensive 29-color flow cytometry panel for human peripheral blood NK cells.
  • To enable in-depth characterization of NK cell differentiation, cytotoxicity, tissue residency, and dysfunction.
  • To facilitate preclinical development and monitoring of NK cell-based therapies.

Main Methods

  • Development and optimization of a 29-color flow cytometry panel.
  • Utilized freshly isolated and ex vivo activated NK cells from human peripheral blood mononuclear cells (PBMCs).
  • Included markers for NK cell differentiation, cytotoxicity, tissue residency, and dysfunction.

Main Results

  • A validated 29-color flow cytometry panel for comprehensive human NK cell analysis.
  • Demonstrated utility in characterizing both resting and activated NK cell populations.
  • Identified key markers for assessing NK cell status relevant to therapeutic applications.

Conclusions

  • The developed flow cytometry panel provides an extensive tool for human NK cell characterization.
  • This panel supports standardized evaluation of therapeutic NK cell products.
  • Facilitates advancements in preclinical development and monitoring of NK cell therapies.

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