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Updated: Sep 19, 2025
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
Published on: January 13, 2023
OMIP-115: High-Dimensional Phenotypic Characterization of Human Natural Killer Cells for Therapeutic Use
Emil Birch Christensen1, Moritz Schaefer1, Mike Bogetofte Barnkob1
1Centre for Cellular Immunotherapy of Haematological Cancer Odense (CITCO), Department of Clinical Immunology, Odense University Hospital, University of Southern Denmark, Odense, Denmark.
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This 29-color flow cytometry panel was developed and optimized for in-depth characterization of human peripheral blood NK cells for preclinical development and monitoring of NK cell therapies. The panel includes markers associated with NK cell differentiation, cytotoxicity, tissue residency, as well as NK cell dysfunction. Panel optimization was performed on freshly isolated and ex vivo activated NK cells enriched from human peripheral blood mononuclear cells (PBMCs). Overall, this panel functions as a tool to extensively characterize human NK cells, paving the way for rapid and standardized approaches to evaluate the biological activity of therapeutic NK cell products.
