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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

Sox9 regulates Papss2 mRNA expression through derepression of the transcriptional repressor, C/EBPβ, in chondrogenic

Cunren Liu1, Rosa Serra1

  • 1Department of Cell Developmental and Integrative Biology, University of Alabama at Birmingham.

Gene Reports
|June 2, 2025
PubMed
Abstract

Insights

Sox9 regulates Papss2 gene expression by interacting with C/EBPβ, preventing its DNA binding and reducing its levels. This mechanism derepresses C/EBPβ-inhibited Papss2 transcription.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Chondrogenesis

Background:

  • Sox9 is a key regulator of chondrogenesis.
  • Previous studies indicated Sox9 regulates Papss2 expression in chondrocytes.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which Sox9 regulates mouse Papss2 mRNA expression.

Main Methods:

  • Luciferase reporter assays in ATDC5 cells.
  • Electromobility shift, competition, and super shift assays.
  • Western blot and co-immunoprecipitation assays.

Main Results:

  • Identified a conserved Sox9-responsive element in the Papss2 gene.
  • C/EBPβ acts as a transcriptional repressor, inhibiting Sox9-mediated activation.
  • Sox9 interacts with C/EBPβ, reducing its DNA binding and overall protein levels.

Conclusions:

  • Sox9 derepresses C/EBPβ-inhibited Papss2 transcription.
  • Sox9 prevents C/EBPβ binding to DNA and reduces C/EBPβ expression.
Keywords:
ATDC5C/EBPβPapss2Sox9cartilagechondrocytetranscription

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