N-Pep-12 Improves Hippocampal Cognitive Function and Increases Neuroplasticity and Neurogenesis Markers

Area of Science:

• Neuroscience
• Gerontology
• Pharmacology

Background:

• Aged mice (129S1/SvImJ) exhibit age-associated cognitive impairments.
• Neuroprotective dietary supplements are being explored to mitigate cognitive decline.
• Hippocampal plasticity and neurogenesis are crucial for memory and learning.

Purpose of the Study:

• To investigate the effects of N‑PEP‑12 on cognitive function, neuroplasticity, and neurogenesis in aged mice.
• To determine if N‑PEP‑12 can improve memory retention and enhance neural health.
• To elucidate the molecular mechanisms underlying N‑PEP‑12's cognitive effects.

Main Methods:

• Chronic administration of N‑PEP‑12 or Vehicle to aged mice.
• Cognitive performance assessment using cued fear conditioning (CFC) and object location memory (OLM) tests.
• In vitro analysis of neuroplasticity markers (NF‑L expression, AVP promoter methylation) and blood-brain barrier integrity.

Main Results:

• N‑PEP‑12 significantly improved associative and contextual memory in CFC and OLM tests.
• In vitro studies showed N‑PEP‑12 increased NF‑L expression and decreased AVP promoter methylation.
• N‑PEP‑12 preserved blood-brain barrier integrity under oxidative stress, suggesting protection against vascular cognitive decline.

Conclusions:

• N‑PEP‑12 promotes hippocampal health by enhancing neurogenesis and neuroplasticity.
• The supplement shows potential in mitigating age-related cognitive decline.
• N‑PEP‑12 is a promising agent for supporting cognitive function in aging populations via neurobiological modulation.