Examinations of cerebrospinal fluid immunoglobulin G by chromatofocusing

IgG components in the CSF and paired sera were examined by chromatofocusing. The separations were performed using the FPLC system with a mono P column and were based on a pH gradient between 9.5 and 6.0. A total amount of 0.1 mg IgG was applied to the column. All samples with oligoclonal IgG were easily identified and the findings differed clearly from those of normal IgG. The sensitivity, resolution capacity and relatively fast as well as easy performance make chromatofocusing a promising tool for investigations of IgG in the CSF.