Identification and Validation of RPL7 and RCCD1 as Potential Biomarkers Associated with Immune Infiltration in...

Area of Science:

• Reproductive Biology
• Genomics
• Immunology

Background:

• Thin endometrium (TE) is a significant cause of embryo implantation failure and female infertility.
• The underlying molecular mechanisms and genetic factors contributing to TE remain largely unknown, hindering effective treatment development.

Purpose of the Study:

• To identify novel genes associated with TE through comprehensive transcriptomic analysis.
• To explore the potential roles of identified genes in TE pathogenesis and immune dysregulation.
• To validate candidate biomarkers for TE diagnosis and therapeutic targeting.

Main Methods:

• RNA sequencing was performed on 18 thin endometrium and 18 normal endometrium samples.
• Bioinformatics analyses, including differential gene expression and functional enrichment, were employed.
• Candidate biomarkers were validated at mRNA and protein levels, and immune infiltration patterns were analyzed.

Main Results:

• Ribosomal protein L7 (RPL7) and RCC1 domain-containing 1 (RCCD1) were identified as significantly overexpressed in TE samples.
• These genes were found to be associated with altered immune cell infiltration and immune regulation.
• RPL7 and RCCD1 demonstrated potential as specific biomarkers for thin endometrium.

Conclusions:

• RPL7 and RCCD1 emerge as promising biomarkers for thin endometrium, offering diagnostic potential.
• The identified genes may play a role in the immune-related pathogenesis of TE.
• Targeting RPL7 and RCCD1 could lead to novel therapeutic strategies for improving clinical outcomes in infertile patients with TE.