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Updated: Sep 19, 2025

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

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Published on: October 6, 2014

Native Mass Spectrometry Facilitates Hit Validation in DNA-Encoded Library Technology.

Philipp Bittner¹, Andreas Gloger², Michelle Keller²

¹Laboratory of Organic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, 8093, Switzerland.

Angewandte Chemie (International Ed. in English)

|June 3, 2025

Summary

This summary is machine-generated.

DNA-encoded Library (DEL) technology speeds drug discovery. Native mass spectrometry now rapidly validates DEL hits by analyzing protein-ligand interactions without labeling, accelerating medicinal chemistry.

Keywords:

DNA‐encoded libraries Drug discovery Hit validation Native mass spectrometry Protein–ligand interactions

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Area of Science:

Biochemistry
Drug Discovery
Analytical Chemistry

Background:

DNA-encoded Library (DEL) technology is a powerful tool in drug development, enabling screening of billions of compounds.
Validating and characterizing hits from DEL selections is a significant bottleneck due to time-consuming processes.

Purpose of the Study:

To introduce native mass spectrometry as a method for rapid validation of On-DNA compounds from DEL screens.
To streamline the hit identification and characterization process in DEL technology.

Main Methods:

Utilizing native mass spectrometry to analyze noncovalent complexes of proteins and On-DNA ligands in the gas phase.
Analyzing purified On-DNA binders and unpurified mixtures to assess binding and rank affinities.

Main Results:

Successfully demonstrated the ability to rank affinities of purified On-DNA binders.
Showcased the capability to detect binding of On-DNA compounds from unpurified mixtures, reducing purification needs.
Provided insights into the binding contributions of different molecular moieties.

Conclusions:

Native mass spectrometry offers a rapid and efficient method for hit validation in DEL technology.
This approach accelerates medicinal chemistry efforts by simplifying hit characterization and providing early insights into structure-activity relationships.