Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

1.4K
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
1.4K
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

2.5K
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
2.5K
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

80.0K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
80.0K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

69.5K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
69.5K
Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

2.3K
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
2.3K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing Outcome Measurement in Oncology Clinical Trials Through Artificial Intelligence: A Scoping Review.

JCO clinical cancer informatics·2026
Same author

Olaparib in HR-deficient, metastatic triple-negative breast and platinum-sensitive relapsed ovarian cancers without germline mutations in BRCA1/2: phase 2 EMBRACE trial.

British journal of cancer·2026
Same author

Recurrent intra-tumour heterogeneity is a hallmark of metastatic prostate cancer.

Nature communications·2026
Same author

The Landscape of Prostate Tumour Methylation.

Cancer discovery·2026
Same author

The transmembrane domain structure of TNFR1 suppresses ligand-independent autoactivation but is not required for TNF-induced signaling.

Science signaling·2026
Same author

Membrane-Based Assembly and Interactions in Immune Receptors.

Chemical reviews·2026

Related Experiment Video

Updated: Mar 31, 2026

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides
07:19

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

1.6K

Resolution of a T1-Like Bacteriophage Outbreak by Receptor Engineering.

Katrina A Black1,2,3, Julie V Nguyen1, Jolene R Ramsey4

  • 1The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.

Molecular Biotechnology
|June 3, 2025
PubMed
Summary

Bacteriophage contamination halted protein production. Scientists developed a CRISPR-based method to generate Escherichia coli resistance against LptD-dependent phages, preventing future outbreaks.

Keywords:
Bacteriophage receptorBacteriophage resistanceCRISPR/Cas9LptDReceptor engineeringSiphophage

More Related Videos

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

13.1K
Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

836

Related Experiment Videos

Last Updated: Mar 31, 2026

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides
07:19

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

1.6K
Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

13.1K
Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

836

Area of Science:

  • Microbiology
  • Molecular Biology
  • Structural Biology

Background:

  • Bacteriophage contamination poses significant risks to biomolecular production, especially when the phage is unidentified.
  • Escherichia coli culture lysis in a structural biology lab halted protein production, even with T1-resistant strains.

Purpose of the Study:

  • To identify the causative agent of Escherichia coli culture lysis.
  • To determine the host receptor of the identified lytic coliphage.
  • To develop a method for generating phage resistance in Escherichia coli.

Main Methods:

  • Genetic analysis of the isolated phage.
  • Transmission electron microscopy.
  • Sequence and structural modeling of the putative receptor-binding protein.
  • Targeted genomic LptD loop deletion using CRISPR technology.

Main Results:

  • The isolated phage was identified as a highly virulent lytic coliphage, vB_EcoS_OzMSK, closely related to Rtp-like siphophages.
  • Sequence and structural analysis suggested LptD as the phage's terminal receptor.
  • A CRISPR-based genomic LptD loop deletion successfully conferred resistance to vB_EcoS_OzMSK in Escherichia coli BL21(DE3) without affecting fitness.

Conclusions:

  • LptD is the host receptor for the virulent coliphage vB_EcoS_OzMSK.
  • Targeted genomic modification of LptD provides a viable strategy for generating phage resistance.
  • A CRISPR-based, single-plasmid solution can prevent future LptD-dependent lytic phage outbreaks in research facilities.