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Chemical Triphosphorylation of Oligonucleotides

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Published on: June 2, 2022

An RNA Chemical Phosphorylation Reagent Based on the "DMT-On" Strategy

Coelio Vallée¹, Jawad Belkheir¹, Nina Gonnet¹

¹Département de Chimie Moléculaire, Université Grenoble Alpes, CNRS UMR 5250, 38000 Grenoble, France.

Organic Letters

|June 3, 2025

Abstract

None:

We present a new phosphorylation reagent built around a dihydroxyacetone core. DNA and, more interestingly, RNA can be easily phosphorylated by using a protocol similar to that routinely employed in the synthesis of unmodified nucleic acids. The well-known advantages associated with the dimethoxytrityl group (on-resin monitoring by trityl cation assay, delayed retention on HPLC, simple deprotection steps, and the use of equipment commonly found in oligo laboratories) are thus maintained.

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