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Published on: June 3, 2019
Rapid identification of a Serratia marcescens outbreak in a neonatal intensive care unit by third-generation
Desiree Henares1,2,3, Meritxell Cubero4, Irene Martinez-de-Albeniz5
1Department of RDI Microbiology, Hospital Sant Joan de Déu, Esplugues de Llobregat, Barcelona, Spain.
Background:
Serratia marcescens is a frequent cause of outbreaks in high-risk hospital settings such as neonatal intensive care units (NICU). This study investigated a potential S. marcescens outbreak in the NICU of a reference children's hospital using Whole Genome Sequencing (WGS). Additionally, it assessed the performance of third-generation sequencing for the rapid and accurate identification and characterization of the outbreak's clonal strain.
Methods:
A prospective study was conducted from September 8th to November 12th 2021, following a sharp increase in invasive S. marcescens infections in the NICU of University Children's Hospital Sant Joan de Déu (Barcelona, Spain). This study included all patients admitted to NICU and other hospital wards from whom S. marcescens was isolated in any sample type. Nanopore sequencing was performed on S. marcescens isolates. Genomic characterization included phylogenetic analyses and detection of antimicrobial resistance genes.
Results:
Twenty-nine patients (16 NICU and 13 non-NICU patients) infected/colonized by S. marcescens were detected during the study period, accounting for a total of 61 isolates. The genomic characterization was performed on 24 isolates from 14 NICU-patients and 10 isolates from eight non-NICU patients. Phylogenetic analyses evidenced three clusters of closely related strains; cluster I (n = 22), II (n = 2) and III (n = 5). The remaining isolates (n = 5) did not cluster. Cluster I contained most isolates from NICU patients (20/24), and most isolates from NICU-patients with confirmed invasive disease (7/8). Cluster II contained two isolates from two NICU-patients, one presenting with invasive disease. The resistance gene blaSRT was found in 97% of S. marcescens isolates (33/34). All isolates exhibited the amikacin-tobramycin aac(6') resistance gene and three multi-drug efflux pumps genes; sdeY, sdeB and smfY. The tetracycline tet(41) resistance gene was found in non-clustered isolates (4/34). The first results were available less than one month after the outbreak's alarm, and complete genomic study after two months.
Conclusion:
Two clonal strains were co-circulating in the NICU setting, with one being the major strain responsible for the outbreak. Rapid molecular characterization with nanopore sequencing confirmed the outbreak. It revealed the phylogenetic relationships among isolates and their antimicrobial potential. This approach enabled effective contextualization of the outbreak and allowed for monitoring its progression.
Insights
Serratia marcescens outbreaks in neonatal intensive care units (NICUs) were investigated using whole genome sequencing. Nanopore sequencing rapidly identified two co-circulating clonal strains, confirming an outbreak and aiding in monitoring.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Serratia marcescens frequently causes hospital outbreaks, particularly in high-risk neonatal intensive care units (NICUs).
- Whole Genome Sequencing (WGS) is crucial for investigating infectious disease outbreaks in healthcare settings.
Purpose of the Study:
- To investigate a potential Serratia marcescens outbreak in a pediatric hospital's NICU.
- To evaluate the efficacy of third-generation sequencing for rapid identification and characterization of outbreak strains.
Main Methods:
- Prospective study of S. marcescens isolates from NICU and other wards.
- Nanopore sequencing for genomic characterization, including phylogenetic analysis and antimicrobial resistance gene detection.
Main Results:
- Twenty-nine patients were infected/colonized, with 61 isolates identified.
- Phylogenetic analysis revealed three clusters; Cluster I, containing mostly NICU isolates, was linked to invasive disease.
- Key resistance genes (blaSRT, aac(6'), efflux pumps) were prevalent, with tet(41) found in non-clustered isolates.
Conclusions:
- Two clonal Serratia marcescens strains co-circulated, with one dominant strain causing the NICU outbreak.
- Rapid nanopore sequencing confirmed the outbreak, elucidated strain relationships, and identified antimicrobial resistance profiles.
- This genomic approach facilitated effective outbreak management and monitoring.
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