Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From Fundamental Photophysics to Photocatalysis: Energy Gap Law Analysis of Anion Radical Excited States.

ACS central science·2026

Same author

A human induced pluripotent stem cell model for the holistic study of epithelial-to-mesenchymal transitions.

Nature methods·2026

Same author

Multi-omic profiling of human antibody-secreting cells reveals diverse subsets sustain durable humoral immunity.

bioRxiv : the preprint server for biology·2026

Same author

Cell shapes decode molecular phenotypes in image-based spatial proteomics.

Cell systems·2026

Same author

A framework for the exploration of subcellular compartmentalization of RNA-binding proteins.

Nature communications·2026

Same author

A high-resolution spatial map of cilia-associated proteins in the human fallopian tube.

Nature communications·2026

Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026

Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026

Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026

Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026

Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026

Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 15, 2025

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

Cell shapes decode molecular phenotypes in image-based spatial proteomics.

Trang Le^1,2, William D Leineweber¹, Matheus P Viana³

¹Department of Bioengineering, Stanford University, Stanford, CA, USA.

Biorxiv : the Preprint Server for Biology

|June 4, 2025

Summary

This summary is machine-generated.

Cell shape significantly influences protein behavior and cellular fate, even within the same cell cycle phase. This study reveals how proteome organization relates to cell geometry, impacting cell function and response to drugs.

More Related Videos

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

Related Experiment Videos

Last Updated: Jun 15, 2025

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

Area of Science:

Cell Biology
Biophysics
Proteomics
Bioinformatics

Background:

Cellular and tissue diversity arises from basic cell shapes and biophysical constraints on cytoskeletal organization.
Cellular geometry is linked to biological processes like polarity, signaling, and morphogenesis.
The relationship between the entire proteome and cell shape is not well understood.

Purpose of the Study:

To perform an integrated analysis of organelle, pathway, and single protein levels in association with a 2D cellular shapespace.
To understand the orchestration of the whole proteome in relation to cell shape.
To analyze protein spatial localization shifts under drug perturbation using a shape-defined coordinate framework.

Main Methods:

Utilized over 1 million images of single cells from the Human Protein Atlas database.
Analyzed 11,998 proteins across 10 cell lines.
Integrated analysis of organelle, pathway, and single protein levels within a 2D cellular shapespace.

Main Results:

Cell and nuclear shapes form a shared continuum across cell lines.
Subcellular organelle topology varies by cell line but is robust within each cell line's shapespace.
Cells of different shapes in the same cell cycle phase may prepare for different fates; many non-cell cycle proteins show shape-based abundance variation.

Conclusions:

Cell shape is a critical determinant of proteome organization and cellular function.
Protein localization and abundance are significantly influenced by cell geometry.
A shape-based coordinate framework enables analysis of drug perturbation effects on protein localization.