Improving proteomic dynamic range with Multiple Accumulation Precursor Mass Spectrometry (MAP-MS)

Area of Science:

• Analytical Chemistry
• Mass Spectrometry
• Proteomics

Background:

• Orbitrap (OT) mass spectrometers offer high-resolution analysis but suffer from slower scanning speeds compared to other mass analyzers.
• Existing methods for dynamic range extension in OT-MS are limited.

Purpose of the Study:

• To improve the precursor dynamic range of Orbitrap mass spectrometers.
• To introduce a novel method for enhancing precursor ion quantification without hardware or software modifications.

Main Methods:

• Utilized long Orbitrap transients to extend precursor dynamic range.
• Developed Multiple Accumulation Precursor Mass Spectrometry (MAP-MS) by multiplexing several precursor m/z ranges into a single scan.
• Collected data using both data-dependent acquisition (DDA) and data-independent acquisition (DIA) methods.

Main Results:

• MAP-MS achieved nearly 2x dynamic range extension with no adverse effects.
• Precursor quantification improved with higher quality measurements in DDA.
• DIA detection sensitivity was enhanced by up to 11% when combining precursor and tandem mass spectra.

Conclusions:

• MAP-MS is an effective strategy for enhancing precursor dynamic range in Orbitrap mass spectrometry.
• The method offers significant benefits for both DDA and DIA workflows, particularly in peptide detection and quantification.